An integrated metabolomic and proteomic approach for the identification of covalent inhibitors of the main protease (Mpro) of SARS-COV-2 from crude natural extractsArticle Published on 2023-01-152022-11-15 Journal: Talanta [Category] SARS, 유전자 메커니즘, 치료제, [키워드] Analysis applied approach baicalein binding carried catalytic characteristic Chinese complementary complex Compound Covalent binder Cys145 database drug target electrophilic Final functional HIS163 HIS164 His41 independent inhibitor inhibitors Ion knowledge M pro M(pro) mass mass spectrometry maturation metabolomic metabolomics Michael molecular dynamics MPro nucleophilic peptide platform polyprotein protease protein-ligand Proteins proteomic proteomics residue SARS-CoV-2 SARS-CoV-2. Scutellaria baicalensis tested the SARS-CoV-2 [DOI] 10.1016/j.talanta.2022.123824 PMC 바로가기
Temporal dynamics of SARS-CoV-2 genome and detection of variants of concern in wastewater influent from two metropolitan areas in ArkansasArticle Published on 2022-11-252022-11-15 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] Affect approach Arkansas association Asymptomatic changes in Community complementary contribute coronavirus COVID-19 COVID-19 case COVID-19 testing death detect diagnostic Factors fecal genetic diversity Genetic variant genomic change highlight identity infected individual investigated nucleocapsid ORF1ab polymerase chain polyprotein public health quantified Quantitative recorded RNA sequencing RT-qPCR RT-qPCR assay SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 mutations SARS-CoV-2 RNA SARS-CoV-2 variant sensitivity silent Support Surface glycoprotein Surveillance TaqMan assay target Temporal the disease transmission of SARS-CoV-2 variants variants of concern viral gene viral titer virus virus genome was used wastewater Wastewater. [DOI] 10.1016/j.scitotenv.2022.157546 PMC 바로가기
Diastereomeric Resolution Yields Highly Potent Inhibitor of SARS-CoV-2 Main ProteaseArticle Published on 2022-10-132022-11-15 Journal: Journal of medicinal chemistry [Category] COVID19(2023년), SARS, 치료제, [키워드] 3CL pro administration antiviral activity Antiviral treatment binding cell types complexes COVID-19 COVID-19 pandemic crystal structure enzyme inactive inhibitor M pro polyprotein potent protease Resolution SARS-CoV-2 translated viral replication Viral RNA yield [DOI] 10.1021/acs.jmedchem.2c01131 PMC 바로가기
Structural and functional characterization of NEMO cleavage by SARS-CoV-2 3CLproArticle Published on 2022-09-082022-11-15 Journal: Nature Communications [Category] SARS, 진단, 치료제, [키워드] 3CLpro accounted addition binding cleavage cleave computational method COVID-19 Efficiency Essential explain functional help Host Host immune response humans Hydrogen bond hydrophobic immune in viral In vitro assay information machine modulator NEMO NF-κB pathology polyprotein predict protease Proteins residue reveal SARS-CoV-2 Signaling specificity substrate the SARS-CoV-2 Variation [DOI] 10.1038/s41467-022-32922-9 PMC 바로가기
X-ray crystallographic characterization of the SARS-CoV-2 main protease polyprotein cleavage sites essential for viral processing and maturationArticle Published on 2022-09-032022-11-16 Journal: Nature Communications [Category] SARS, 변종, [키워드] Analysis Antiviral binding C-terminal C-terminally capture catalytic cause cleavage cleavage site cleavage sites Complete complex coronavirus 2 COVID-19 enzyme event form Interaction M pro maturation mutant non-structural protein pathogen plasticity polyprotein polyproteins Prevent protease provide representing respiratory responsible SARS-CoV-2 specificity substrates the SARS-CoV-2 therapeutic unique X-ray X-ray crystallographic structure [DOI] 10.1038/s41467-022-32854-4 PMC 바로가기
Structural investigations of proteins encoded by SARS-CoV-2Article Published on 2022-09-012022-11-16 Journal: FEBS Open Bio [Category] SARS, 변종, 유전자 메커니즘, [키워드] antibodies articles bases categories cellular cleaved commenced coronavirus COVID COVID-19 pandemic cysteine proteases described ENCODE Evolution example expressed Final Genome include knowledge MN908947.3 molecular mechanism other coronaviruses pandemic polyprotein positive-sense Protein protein structure purified reported Research ribonuclease RNA genome RNAs SARS-CoV-2 selected sequenced single-stranded small molecule drugs Spike protein spike protein. structural property the disease the spike protein translation Vaccines variant viral transcript were expressed [DOI] 10.1002/2211-5463.13465 PMC 바로가기
A robust high-throughput fluorescence polarization assay for rapid screening of SARS-CoV-2 papain-like protease inhibitorsSARS-CoV-2 파파인 유사 프로테아제 억제제의 신속한 스크리닝을 위한 강력한 고처리량 형광 편광 분석Article Published on 2022-09-012022-09-11 Journal: Virology [Category] COVID19(2023년), SARS, 치료제, [키워드] Anacardic acid antiviral agent Antiviral agents cleavage competitive inhibitor COVID-19 develop disease effective therapy Fluorescence Fluorescence polarization functional High-throughput screening immune evasion in vitro inhibitor inhibitors lifecycle Papain Papain-like protease Papain-like protease inhibitors pathogen PLPro polyprotein protease protease inhibitors public health responsible robust SARS-CoV-2 SARS-CoV-2. Vaccines [DOI] 10.1016/j.virol.2022.07.006 PMC 바로가기 [Article Type] Article
New Perspectives on Antimicrobial Agents: Molnupiravir and Nirmatrelvir/Ritonavir for Treatment of COVID-19항균제에 대한 새로운 관점: 코로나19 치료를 위한 몰누피라비르 및 니르마트렐비르/리토나비르Article Published on 2022-08-162022-09-11 Journal: Antimicrobial Agents and Chemotherapy [Category] COVID19(2023년), SARS, 변종, 치료제, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus addition adjusted relative risk antimicrobial Antiviral cause childbearing potential children Complication Contraception coronavirus disease Coronavirus-2 COVID-19 cytochrome P450 Cytochrome P450 enzymes death drug Drug administration Drug-to-drug interaction Each Emergency use authorization food hospitalizations in vitro inhibit limit management medication metabolism Mild mild to moderate moderate molnupiravir morbidity and mortality Mutation New Nirmatrelvir nirmatrelvir/ritonavir. nonspecific outcome pandemic Patient polymerase polyprotein pregnant protease proteolytic cleavage reduction Relative risk Replication Respiratory disease risk Ritonavir SARS-CoV-2 Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus severe acute respiratory syndrome coronavirus-2 significantly target tested Toxicity Treatment treatment regimen U.S. Food and Drug Administration variant viral replication virus virus production [DOI] 10.1128/aac.02404-21 PMC 바로가기 [Article Type] Article
Genetically Encoded Fluorescent Sensors for SARS-CoV-2 Papain-like Protease PLproSARS-CoV-2 Papain-like Protease PLpro용 유전적으로 인코딩된 형광 센서Article Published on 2022-07-152022-09-11 Journal: International Journal of Molecular Sciences [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] acceptor Biliverdin Cell cleavage co-expression coronavirus COVID-19 detect Donor downstream Endoplasmic reticulum energy transfer enzymatic activity fluorescence intensity fluorescent fluorescent protein FRET genetically encoded probes HeLa cells increase in inhibitors lifecycle linker live cell live cell imaging live cells membrane mNeonGreen non-structural protein non-structural proteins nsp1 NSP2 Nsp3 Papain Papain-like protease PLPro polyprotein protease protease PLpro Protein SARS-CoV-2 SARS-CoV-2 propagation sensor separated Stage the SARS-CoV-2 translocation translocation. [DOI] 10.3390/ijms23147826 PMC 바로가기 [Article Type] Article
Paxlovid: Mechanism of Action, Synthesis, and In Silico StudyPaxlovid: 작용 메커니즘, 종합 및 인 실리코 연구Review Published on 2022-07-072022-09-11 Journal: BioMed Research International [Category] COVID19(2023년), SARS, 치료제, [키워드] Action antiviral activity antiviral drug antiviral efficacy antivirus antiviruses Biochemistry candidate cleave clinical trial Combination coronavirus COVID COVID-19 COVID-19 pandemic described description drug flexible Glutamine Human coronavirus Immune profile in vitro in vivo inhibiting inhibitor mechanism mechanism of action molnupiravir Nirmatrelvir Organic chemistry pandemic Paxlovid PF-07321332 Pfizer Pharmacology polyprotein protease Protease inhibitor Proteases replaced REPLACED BY reported Ritonavir SARS-CoV-2 protease synthesis Treatment treatment of COVID-19 Vaccine viral replication [DOI] 10.1155/2022/7341493 PMC 바로가기 [Article Type] Review